- Beckman coulter ampure xp manual#
- Beckman coulter ampure xp software#
- Beckman coulter ampure xp series#
- Beckman coulter ampure xp free#
Beckman coulter ampure xp free#
Both procedures were shown to be free of cross-contamination between wells.įigure 2. Efficient and reproducible automated AMPure XP purification can be completed in less than 90 minutes for 192 PCR samples (10 – 70 μL).
Beckman coulter ampure xp manual#
Amplification of a test template was highly efficient and reproducible, with yields equivalent to manual amplification. One to 192 PCR sample reactions can be setup in two 96-well PCR plates using up to two plates of master mixes, two primer sources and two sample sources, including master mix made from individual components.
Beckman coulter ampure xp software#
The pipetting tools, updated software and Windows 7 compatibility improve the ease and speed of reaction setup. This application note describes the use of the Biomek 4000 Laboratory Automation Workstation to automate PCR reaction setup, as well as amplicon purification using the AMPure XP system. Requiring no centrifugation or vacuum filtration, the AMPure XP reagent system is highly amenable to automation. The excess primers, nucleotides, salts and enzymes from the reaction are removed using a simple Bind-Wash-Elute procedure (Figure 1). The AMPure XP reagent system uses an optimized buffer to selectively and reversibly bind PCR amplicons that are 100bp and larger to solid-phase paramagnetic beads. While many purification systems are commercially available, the Agencourt AMPure XP reagent system is a highly efficient, easily automated PCR purification system that delivers superior quality DNA with no salt or dNTP carryover. Automation of reaction setup can also eliminate both human error and contamination associated with manual processing, while assuring high quality results.Įfficient recovery of pure PCR amplicons is essential for downstream applications such as genotyping, cloning and primer walking. Given the number of samples analyzed by PCR, the multitude of amplification formats used, and the need to reduce assay costs, a simple, easy-to-use, and high-throughput automation solution for PCR reaction setup is absolutely necessary. Schematic representation of the workflow for the six-step automated amplicon purification procedure. It is now used in a wide range of formats, including RT-PCR, qPCR, Multiplex PCR, Inverse PCR, digital PCR, and Miniprimer PCR.įigure 1. Its exceptional ability to accurately amplify and quantify extremely low levels of nucleic acids has driven the emergence of numerous applications, including genetic testing, tissue typing, infectious disease identification, genetic fingerprinting, DNA sequencing and cloning, and gene expression. Polymerase chain reaction (PCR) is a fundamental and essential technique for medical and biological research, as well as forensic identification. Both procedures were demonstrated to be free of cross-contamination. In addition, the Agencourt AMPure XP reagent system has been automated on the Biomek 4000 Workstation to provide rapid amplicon purification (two 96-well plates in less than 90 minutes) with yield and purity equivalent to manual purification.
The Biomek 4000 Laboratory Automation Workstation has been used to demonstrate highly reproducible real-time amplification (CV<2%) after automated reaction setup. Ruth Zhang, Li Liu, Amy Yoder, John Palys, Bryan Daniels, Isabel Gautreau and William Godfreyīeckman Coulter Life Sciences, Indianapolis, IN Abstract
Beckman coulter ampure xp series#
0 kommentar(er)
